A. In the subcutaneous model of A375- CD1d, upon injection of iNKT cells, cells infiltrated their natural homing site, bone marrow, whereas some of the iNKT cells made it in the tumor. Over time, iNKT cells residing in blood and bone marrow of mice did not expand, whereas the iNKT cells in the tumors expanded over time.
B. Only
the iNKT cells that reached the tumor became activated in response to their environment but not the cells in the blood or bone marrow.
C. Whereas the iNKT cells in the blood or bone marrow cannot maintain their proliferative capacity,
proliferation of iNKTs in the tumor increases (Representative plots from tumor samples)